Major fatty acids were iso-C16:0 and C17:1П‰8c. The predominant menaquinones were MK-9(H6) and MK-9(H4). The polar lipid profile contained phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylglycerol, glycophospholipid, amino-phospholipid and two unidentified phospholipids. Cell-wall sugars were composed of arabinose, fucose, glucose, mannose, rhamnose and xylose. Whole-cell hydrolysates of strain NA12T contained 3-hydroxydiaminopimelic acid and meso-diaminopimelic acid. The G + C content of genomic DNA was 71.5%. Digital DNAвЂ“DNA hybridization values between strain NA12T and the closely related type strains were ranged from 41.0 to 18.3% while the average nucleotide identity values were between 87.3 and 86.5%, which are well below the designed cut-off points of 70 and 95%, respectively. endophytica 202201T with pairwise sequence identity values ranging from 99.4 to 99.3%. The 16S rRNA gene sequence analysis of strain NA12T showed that the strain closely related to M. Cultural and chemotaxonomic characteristics of the strain were consistent with its classification within the family Micromonosporaceae. The taxonomic position of the strain was established using a polyphasic approach. craniellae LHW63014T to produce a wide variety of novel antibiotics, especially for metal ion-chelating agents.ДЄ novel actinobacterial strain, designated NA12T, was isolated from coastal sediment sample of Nemrut Lake, a crater lake in eastern Anatolia, Turkey. craniellae LHW63014T, 19 of which showed low similarity (<50%) to known BGCs and 5 of which showed the closest homology with BGCs encoding metal ion-chelating agents, indicating the immense potential of M.

Secondary metabolites prediction using antiSMASH revealed that 22 biosynthetic gene clusters (BGC) of secondary metabolites were located in the genome of M. Genomic annotations revealed that 79.09% of the protein-coding genes were assigned to the COG database, among which, the abundant genes were predicted to be involved in transcription, replication, recombination and repair, and amino acid transport and metabolism. The complete genome contained 6572 protein-coding genes, 48 tRNA genes, and 9 rRNA genes. craniellae LHW63014T, which is comprised of a circular chromosome of 6,839,926 bp with the G + C content of 70.9 mol%. In this study, we report the complete genome sequence of M. Micromonospora craniellae LHW63014T is a novel marine Micromonospora, isolated from a Craniella species sponge collected in the South China Sea. endophytica be transferred to genus Micromonospora as Micromonospora endophytica comb. Based on phenotypic characteristics, phylogenetic data and digital DNA-DNA hybridization results, strain LHW63014T could be distinguished from its closest taxa, representing a novel species of the genus Micromonospora, for which the name Micromonospora craniellae sp.

Morphological and chemotaxonomic characteristics confirmed their affiliation to the genus Micromonospora. endophytica 202201T located within the genus Micromonospora. Phylogenetic analyses of the family Micromonosporaceae based on the 16S rRNA and gyrB gene sequences indicated that strain LHW63014T and J.

The 16S rRNA gene sequence of strain LHW63014T showed highest similarities to Jishengella endophytica 202201T (98.9 %), Micromonospora olivasterospora DSM 43868T (98.7 %), Micromonospora maris AB-18-032T (98.6 %) and Micromonospora gifhornensis DSM 44337T (98.6 %). A novel marine actinomycete, designated LHW63014T, isolated from a marine sponge, Craniella species, collected in the South China Sea, was examined using a polyphasic approach.

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